graham
New member
mmilner said:John, can u enlarge on this? Eg:- What OBA's, where can you get them from? Are they non-toxic? Where would I get non fluorescent cotton wool? (Oh, I think someone mentioned tampons above, if so, then cool, I will buy some and check them with my UV light, lol.)
They'd better be non-toxic 'cos you'll find them in Persil etc.
mmilner said:Would it then fluoresce under a UV light, (guess they would), which I have got. Of course, using a non-visual method would require "pre-test detectors (before the OBA is injected) to see if the anticipated risings are contaminated with fluorescent reagents from other sources." Any info would be gratefully received. Ta.
Aye, the water is adsorbed onto cotton wool and the wool will then fluoresce under UV. So do your whites after a wash, check them out. And that's the problem, all you need is one washing machine discharging somewhere in your catchment & you are stymied. Yes, you can check for background fluorescence beforehand, but that's when the guys in that remote cottage were on holiday and didn't do any washing! I would not trust the method anywhere except wholly deserted uplands, like where Pitlamp lives
mmilner said:Also, " has the advantage of not requiring laboratory reagents to process the detectors". Tell me more! It might be on the page that Leclused posted so apols if so. Not had chance to read it fully yet.
Regards, Mel.
Think Leclused's links were about dye not OBAs. The advantage of OBAs is that no post-treatment is required, just shine the UV at the cotton wool pads.
To cover a couple of other points:
Lycopodium spores are a terrifically labour intensive method and quite finicky.
The other advantages of using dyes is that you can use multiple dyes (though, as Bograt notes you have to be careful, some of them can be pretty nasty) and using a fluorimeter can pick them up individually from your detectors thus enabling multiple traces to be done at once and it is possible, given the resources, to take a punt at quantitative as well as qualitative tracing.
The one thing to beware of, of course, is that some other bugger, water authority, researcher or whatever, isn't also doing a similar trace somewhere nearby, testing for a leak or something & screwing with your results. This is why it is in your interest (as well as theirs) to clear all your traces with the relevant local water authorities in advance. They should know.
And on the subject of screwing up, don't pollute your own detectors! When dye tracing in Ireland, the UBSS used to have a 'dirty' car which carried dye & whose passengers were responsible for injecting it and a 'clean' car whose passengers were responsible for placing and collecting the detectors. These two teams should be kept apart as much as possible, the stuff cross-contaminates very easily.